Education 9 min read

How the O-Shot Works: A Step by Step Overview

A clear walkthrough of what to expect during the procedure

Before the Procedure

Understanding what happens during an O-Shot procedure can help ease concerns and set realistic expectations. The journey begins well before the actual treatment day, starting with education and consultation.

Initial Consultation Your first appointment focuses on conversation and assessment. A qualified practitioner will discuss your health history, current concerns, and goals. This is your opportunity to ask questions, voice concerns, and determine if this treatment aligns with your wellness objectives.

Medical History Review Your provider will review medications, allergies, and any existing health conditions. Certain medical situations may affect whether the procedure is appropriate for you. Complete honesty during this stage ensures your safety and helps predict likely outcomes.

Setting Realistic Goals During consultation, you will discuss what results might be achievable for your specific situation. Every woman’s body responds differently, and understanding this variation helps create appropriate expectations. Your practitioner should provide honest, balanced information about potential benefits and limitations.

Pre-Procedure Guidelines You may receive specific instructions for the days leading up to your appointment. These might include guidance about hydration, medications to avoid, or other preparations that optimize the procedure’s safety and effectiveness.

The Collection Process

The O-Shot uses platelet rich plasma derived from your own blood, which makes the first step similar to a routine blood draw at your doctor’s office.

Blood Draw A small amount of blood is collected from your arm, typically the same quantity taken during standard laboratory testing. Most women find this step completely familiar and straightforward. The amount collected is enough to create the concentrated plasma needed for treatment.

Your Own Biology One significant advantage of this approach is that the treatment uses components from your own body. This autologous method means the material being used is inherently compatible with your system, reducing concerns about rejection or allergic reactions.

Processing Time After collection, the blood undergoes a centrifuge process. This typically takes about 10 to 15 minutes. During this time, you may relax in the treatment room, ask additional questions, or simply prepare mentally for the next steps.

Preparing the Treatment

While the blood is being processed, preparation for the application stage begins. This involves ensuring your comfort and readiness for the treatment itself.

Numbing Application Most practitioners apply a topical numbing cream to the treatment areas before the procedure. This cream needs time to take effect, usually around 15 to 30 minutes. The goal is to maximize your comfort during the application stage.

Creating the Platelet Rich Plasma The centrifuge separates blood components by density. The platelet rich portion is carefully extracted, creating a concentrated solution containing growth factors and healing properties. This golden colored liquid is what will be used in the treatment.

Quality Verification Practitioners verify that the separated plasma meets quality standards before proceeding. This step ensures that what will be applied contains the appropriate concentration of platelets and beneficial components.

Final Preparation The treatment area is cleansed and prepared according to medical standards. Sterile technique is crucial for safety and optimal results. Your comfort is checked again before moving forward.

The Application Stage

This is the core part of the procedure, where the prepared platelet rich plasma is introduced to specific areas designed to enhance function and sensation.

Precise Placement Using a very fine needle, the practitioner applies the plasma to targeted locations. These sites are chosen based on anatomy and the specific outcomes you are seeking. Precision matters here, as proper placement influences results.

The Experience Thanks to the numbing cream, most women report feeling pressure or awareness rather than significant discomfort. The entire application process typically takes only a few minutes. Many describe it as far more comfortable than they anticipated.

Multiple Injection Sites The treatment involves several small applications to different areas. Each serves a specific purpose in the overall treatment plan. Your practitioner may explain what they are doing as they work, helping you feel informed and in control.

Immediate Completion Once the applications are complete, the procedural portion of your appointment is finished. The entire process from blood draw to completion usually takes about 45 minutes to an hour, though this can vary by practice and individual circumstances.

What Happens After

Understanding the post procedure phase helps you plan appropriately and know what to expect in the hours and days following treatment.

Immediate Aftermath You can typically leave the clinic shortly after the procedure is complete. Most women drive themselves home without difficulty. You may experience some sensitivity in the treatment area, but this is usually mild and temporary.

First 24 Hours Some initial swelling or tenderness is normal and expected. Your practitioner will provide specific guidance about what is normal versus what should prompt a call to the office. Most women find that any immediate effects are quite manageable.

Activity Guidelines You will receive clear instructions about what activities to avoid and for how long. This typically includes guidance about exercise, intimacy, and other physical activities. Following these recommendations supports optimal healing and results.

Hygiene and Care Proper hygiene is important during the initial healing period. Your practitioner will explain any special care needed and when you can resume normal bathing and personal care routines.

Timeline and Expectations

Results from the O-Shot do not appear instantly. Understanding the timeline helps you maintain realistic expectations and recognize changes as they occur.

Immediate Period In the first few days, you are simply healing. Any results you notice during this time are not the full picture. Patience during this initial phase is important.

Two to Four Weeks Many women begin noticing changes during this window. The growth factors in the plasma are working to stimulate natural regeneration processes. Changes may be subtle at first, then become more apparent.

Full Results Timeline Optimal results typically develop over two to three months. This is because the treatment works by encouraging your body’s own healing and regeneration processes, which take time to fully develop.

Individual Variation Some women notice benefits quickly, while others require more time to experience changes. Factors like age, overall health, and individual biology all influence response time and degree of improvement.

Follow Up Care Your practitioner may schedule follow up appointments to assess progress and address any questions. These check ins are valuable opportunities to discuss your experience and determine if any additional steps might be beneficial.

Duration of Results Results vary by individual, but many women find benefits last anywhere from several months to over a year. Some choose to have repeat treatments to maintain results, while others find a single treatment sufficient.


Understanding each step of the O-Shot process empowers you to make informed decisions about your wellness journey. Always discuss questions and concerns with qualified healthcare providers who can offer personalized guidance based on your unique situation.

The Aesthetic Midwife Monica

Registered Midwife & Advanced Practitioner in Women's Aesthetics

Monica combines her Clinical Labour Ward Midwifery knowledge with the O-Shot to help women feel refreshed, confident, and naturally restored. Registered with the NMC and CMA, Monica provides safe, natural, and empowering treatments for women seeking rejuvenation and confidence.

Registered with the NMC and CMA
20+ years of clinical labour ward midwifery
8+ years leading an approved aesthetics clinic
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